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High-Plex Spatial Biology Analysis Services

Not just what cells express, but where they are located, how they interact, and why they respond differently to treatment.

ORGANOIDSCIENCES’ Spatial Biology Services provide high-plex single-cell spatial analysis to comprehensively characterize disease mechanisms, the tumor microenvironment (TME), and therapeutic responses.

Explore Our Research Cases

Trusted Worldwide

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Qualified Providers Worldwide

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2

Providers in Asia

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1

Qualified Provider in Korea

Officially recognized by Akoya as a Qualified CRO Provider for high-plex spatial biology services.

  • Official Akoya Qualification
  • PhenoCycler™ Fusion Expertise
  • Publication-Ready Spatial Analysis
  • End-to-End Standardized Workflow
  • 100+ Protein Multiplex imaging

Why Choose PhenoCycler™ Fusion for Spatial Biology?

Choose the Right Spatial Biology Technology for Your Research

Different spatial biology technologies answer different biological questions.
PhenoCycler™ Fusion provides industry-leading high-plex protein imaging for cell phenotyping, spatial interaction analysis, biomarker discovery, and drug response evaluation.
  • Feature
    PhenoCycler™ Fusion
    Multiplex IF
    Spatial Transcriptomics
  • Primary Target
    Protein
    Protein
    RNA
  • Spatial Context
    ✓
    ✓
    ✓
  • Single-cell Resolution
    ✓
    Partial
    Near Single-cell*
  • Multiplex Capability
    100+ proteins
    ~8 proteins
    Thousands of genes
  • Best Application
    Cell Phenotyping Tumor Microenvironment (TME) Biomarker Discovery Drug Response Analysis
    Targeted Protein Imaging
    Gene Expression Profiling
* Spatial transcriptomics provides high-resolution gene expression data but does not directly achieve true single-cell protein phenotyping.

How PhenoCycler™ Fusion Works 

Discover More Than Expression 

Go beyond protein expression to understand cellular location, phenotype, and cell-to-cell interactions through automated high-plex spatial protein imaging.

1DNA-Barcoded Antibody Technology

A single staining step enables repeated detection of over 100 protein biomarkers while preserving tissue integrity

2Automated Reveal–Image–Remove Cycles

Automated reveal, image, and remove cycles continuously capture every biomarker with high reproducibility and minimal manual intervention

Diagram of a repeatable lab cycle: reveal, image, remove steps shown on three platforms; a white 'Repeat' arc ties the steps, with a yellow exit arrow and bottom cycle labels (Cycle 1 to Cycle 16+).

3High-Plex Spatial Mapping

Simultaneously analyze cell phenotypes, tissue architecture, tumor microenvironment (TME), and cellular interactions within a single tissue section

End-to-end Workflow

We provide a seamless end-to-end workflow covering sample preparation, multiplex staining, imaging, bioinformatics analysis, and publication-ready reporting.

Sample Submission

Staining

Imaging

Bioinformatics Analysis

Report Delivery

How Spatial Biology Accelerates Your Research

Transform Spatial Data into Biological Insight

Traditional methods often dissociate tissues, removing critical spatial context. Spatial biology preserves tissue architecture, enabling simultaneous analysis of cell location, phenotype, and cell–cell interactions within intact tissue.

Tumor Microenvironment

  • Immune Cell Distribution
  • Immune Hot / Cold Tumor
  • Cell-to-Cell Interaction
  • Tumor Heterogeneity

Target Validation

  • Target Expression
  • Tumor Specificity
  • Spatial Localization
  • Off-target Assessment

Drug Response

  • Drug Response Prediction
  • Mechanism of Action
  • Resistance Analysis
  • Treatment Response Evaluation

Biomarker Discovery

  • Cell Phenotyping
  • Co-expression Analysis
  • Spatial Signature Discovery
  • Novel Biomarker Identification

Explore Our Research Cases

Download real-world case studies and sample reports.
Explore publication-ready reports and analysis results from IO60™, Neuro42™, and Spatial Biology research projects.

Get the Case Report by Email

Multiplex Protein Panels

The PhenoCode™ Discovery IO Human and Mouse Protein Panels provide comprehensive spatial immune profiling for in-depth characterization of the tumor microenvironment (TME). Combined with the Metabolism Panel, they enable integrated analysis of immune phenotypes and metabolic activity at single-cell resolution, supporting advanced biomarker discovery and translational research.

60-marker human IO antibody panel

Immune cell type core

  • CD68HLA-A
  • CD4HLA-DR
  • CD44CD14
  • CD45ROCD20
  • CD45CD3e
  • CD11cCD56
  • CD8

Lymphocyte Profiling

  • CD107aGranzyme B
  • CD57TOX
  • FOXP3TCF-1
  • CD21CD79a
  • CD38CD39

Immune Activation

  • IFNGPD-L1
  • IDO1VISTA
  • PD-1LAG3
  • ICOS

Tissue Architecture

  • CD31Beta-actin
  • E-CadherinPodoplanin
  • SMACollagen IV
  • VimentinCaveolin
  • CD34β-Catenin1

Proliferation

  • Histone H3 Phospho (Ser28)
  • PCNA
  • Ki67

Tumor-Specific Markers

  • Keratin 14Bcl-2
  • SOX2EpCAM
  • PanCKGP100
  • Keratin 8/18TP63
  • ERKeratin 5

Myeloid Profiling

  • iNOSCD11b
  • CD66CD206
  • MPOCD209
  • CD163

24-marker mouse FFPE IO antibody panel

T Cell Markers

  • CD3eT cell
  • CD4Helper T cell
  • CD8Cytotoxic T cell
  • FOXP3Regulatory T cell
  • CD44Activated T cell
  • CD45R/B220Naïve T cell

B Cell Markers

  • CD20B cell

Structural, Proliferation and Tumor Markers

  • Vimentin Mesenchymal cells, epithelial-mesenchymal transition (EMT)
  • Col1A1Extracellular matrix
  • CaveolinIntegral membrane protein
  • PanCKEpithelial cells, tumor marker
  • Ki67Proliferation marker

Other Cell Type Markers

  • CD31Endothelial cells
  • CD36Adipocyte progenitors
  • Ter119Erythroid cells

Tumor-Specific Markers

  • CD68 Macrophage
  • CD206M2 macrophage
  • F4/80Macrophage

Immune Cell Markers

  • CD11cPanleukocyte
  • Ly6gPeripheral neutrophils, monocytes and granulocytes
  • FcRγMultiple immune cell types
  • CD45Nucleated hematopoietic cells
  • Iba1Microglia
  • S100A9Myeloid differentiation

9-marker human metabolism antibody panel

Nutrient Uptake

  • ASCT2GLUT1

PPP / Redox Balance

  • G6PD

Glycolysis / Lactate Metabolism

  • HK1LDHA

Mitochondrial Metabolism / OXPHOS

  • CSIDH2
  • SDHAATP5A
The Human and Mouse Neurobiology Panels enable comprehensive spatial profiling of molecular and cellular changes within brain tissue.
Designed for studies of neurodegeneration, neuroinflammation, and neuroplasticity, they reveal cell-type-specific interactions while preserving tissue architecture and spatial context.

42-marker human neurobiology antibody panel

Neuronal Cell Type Markers

  • Mature NeuronsNeuN
    MAP2
    Neurofilament
    Synaptophysin
  • GABAergic NeuronsGABA
  • Cholinergic NeuronsCHAT
  • Serotonergic NeuronsSerotonin

Neurodegenerative Disease Markers

  • Alzheimer's DiseaseAmyloid-beta
    APOE
    Tau (phospho T181)
    Tau (phospho T231)
    Tau (phospho S396)
    Tau (phospho T205)
  • Parkinson's Disease & SynucleinopathiesAlpha-synuclein
  • General NeurodegenerationH2A.X

Neuroplasticity & Cellular Remodeling

  • Proliferation & NeurogenesisPCNA
    Ki67
  • Astrocyte & Structural PlasiticityS100B
    Vimentin
  • OligodendrocytesOlig-2
    MBP

Blood-Brain Barrier Integrity & Vascular Components

  • Endothelial Cells & Vascular StructureClaudin-5
    CD31
    CD34
    Collagen IV
  • Pericytes & Smooth Muscle CellsSMA
  • Astrocyte-Endothelial InteractionsAQP4

Neuroinflammation & Immune Response

  • MicrogliaTMEM119
  • Activated Microglia & MacrophagesIba1
    CD68
    Mac-2/Galectin-3
    CD11b
    F4/80
    CD206
  • T cell
    Infiltration    CD3e
    CD4
    CD8
  • General
    Immune
    Activation    CD45
    CD44
    CD20
    MHC II
    Ter119
  • Astrocyte- Associated
    Inflammation    GFAP

42-marker mouse neurobiology antibody panel

Neuronal Cell Type Markers

  • Mature NeuronsNeuN
    MAP2
    Neurofilament
    Synaptophysin
  • GABAergic NeuronsGABA
  • Cholinergic NeuronsCHAT
  • Serotonergic NeuronsSerotonin

Neurodegenerative Disease Markers

  • Alzheimer's DiseaseAmyloid-beta
    APOE
    Tau (phospho T181)
    Tau (phospho T231)
    Tau (phospho S396)
    Tau (phospho T205)
  • Parkinson's Disease & SynucleinopathiesAlpha-synuclein
  • General NeurodegenerationH2A.X

Neuroplasticity & Cellular Remodeling

  • Proliferation & NeurogenesisPCNA
    Ki67
  • Astrocyte & Structural PlasiticityS100B
    Vimentin
  • OligodendrocytesOlig-2
    MBP

Blood-Brain Barrier Integrity & Vascular Components

  • Endothelial Cells & Vascular StructureCD31
    Claudin-5
    Col1A1
  • Pericytes & Smooth Muscle CellsSMA
  • Astrocyte-Endothelial InteractionsAQP4

Neuroinflammation & Immune Response

  • MicrogliaTMEM119
  • Activated Microglia & MacrophagesIba1
    CD68
    Mac-2/Galectin-3
    CD11b
    F4/80
    CD206
  • T cell
    Infiltration    CD3e
    CD4
    CD8
  • General
    Immune
    Activation    CD45
    CD44
    CD20
    MHC II
    Ter119
  • Astrocyte- Associated
    Inflammation    GFAP

Elevate the Standard of Your Research

1. Unique Advantage for ADC Research

PhenoCycler-powered spatial profiling enables the simultaneous analysis of drug distribution and target expression within tissue.

In a single experiment, researchers can evaluate target expression, drug distribution, tumor heterogeneity, and spatial biomarker mapping—key factors for advancing ADC development.

Applications

✓ ADC Development

✓ Target Validation

✓ Companion Diagnostics

✓ Translational Research

2. Advanced Sample Processing

The quality of spatial biology data begins with high-quality sample preparation. ORGANOIDSCIENCES provides specialized sample processing expertise for challenging biological specimens, including organoids, 3D tissues, and precious clinical samples, ensuring reliable and reproducible spatial analysis.

Key Benefits

✓ Organoid & 3D Tissue Expertise

✓ Reduced Experimental Failure Risk

✓ High-Quality Multiplex Imaging

3. End-to-End Spatial Analysis

Beyond delivering raw image data, our bioinformatics experts transform complex spatial datasets into publication-ready biological insights.

We provide comprehensive analytical outputs that enable researchers to interpret results and accelerate scientific discovery.

Deliverables

✓ Cell Phenotyping

✓ Cell-to-Cell Interaction Analysis

✓ Neighborhood Analysis

✓ Clustering & Spatial Mapping

✓ Publication-Ready Figures

✓ Comprehensive Analysis Report

4. Ready-to-Use Validated Panels

To reduce the time and cost required for panel development and validation, we provide pre-validated, research-ready multiplex panels.

Researchers can begin projects immediately with optimized panels designed for reproducible and reliable spatial profiling.

Available Panels

✓ IO60™ Immune-Oncology Panel

✓ Neuro42™ Neurobiology Panel

✓ Custom Panel Design Support

Key Benefits

✓ Faster Turnaround Time (TAT)

✓ Reduced Validation Cost

✓ Immediate Project Initiation

✓ Reproducible Results

5. Integrated Analysis Services Connected to the ODISEI™ Platform

Spatial Biology analysis can be integrated with the ODISEI™ Drug Evaluation Platform, allowing a single research project to be expanded into a comprehensive drug evaluation workflow.

Deliverables

✓ Drug efficacy assessment

✓ ADC evaluation

✓ Toxicity assessment

✓ Disease modeling

✓ Multi-omics analysis

6. Trusted Analysis Services for Researchers Worldwide

ORGANOIDSCIENCES supports the entire research process, from experimental execution and data analysis to the delivery of publication-quality results.

Deliverables

✓ High-quality data

✓ Publication-ready reports

✓ Expert bioinformatics analysis

✓ Dedicated scientific support

High-Plex Spatial Biology CRO Services

Reveal how cells are organized, interact, and respond within intact tissue using high-plex spatial biology analysis powered by PhenoCycler™ Fusion.
Talk to Our Expert

Toward a Sustainable Future
with ORGANOIDSCIENCES

Join ORGANOIDSCIENCES in leading groundbreaking organoid research and the advancement of next-generation cell therapies.
Be part of the journey of biomedical innovation that is shaping the future of healthcare.

Technical Inquiry

bd@organoidrx.com

PR/Marketing inquiry

pr@organoidrx.com

Recruitment inquiry

hr.admin@organoidrx.com

General Inquiry

info@organoidrx.com

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